- This is actually the third version of Casino Royale released. The first was a 1954 CBS tv special and the second one was a 1967 spoof.
- The high-stakes Texas Hold'em poker game that was the movie's centerpiece was actually a bacarrat game in the novel and the other film and tv versions.
- Casino Royale was the first James Bond novel written by Ian Fleming.
- Other choices for the role of James Bond included Clive Owen, Hugh Jackman Ewen McGregor and Colin Farrel.
- The delay of four years between Die Another Day and Casino Royale was the second longest behind only Licence to Kill and GoldenEye, which was 6 years
- The "Vesper" that James Bond orders at Casino Royale is taken from the novel. It consists of three measures (9 ounces) of gin (Gordon's was Bond's choice), one measure (3 ounces) of vodka (Bond preferred a grain vodka be used; e.g. Absolut) and half a measure (1.5 ounces) of Kina Lillet. The ingredients are shaken over ice until cold, served in a cocktail glass with a slice of lemon peel for garnish. Kina Lillet has not been made since 1985 and the modern substitute would be Lillet Blanc, made by the same company, Lillet, based in Podensac, France. This also creates somewhat of an anachronism as the film adaptation of Casino Royale is clearly set in the present, where Kina Lillet would no longer be available.
- Casino Royale is also the name of the casino where Bond and Largo face-off in a video game battle in Never Say Never Again.
- The bond girl character name of Solange has previously appeared in two Ian Fleming stories. She was the name of the girl in OO7 In New York and a girl named Solange was referenced in From A View To A Kill.
- A James Bond origin story like this had been touted to be the first Bond film after Roger Moore retired. A first draft screenplay was written where James Bond was a young man in the Royal Navy. However, 'Albert R Broccoli' rejected the concept believing at the time that the audience wasn't really interested in a young James Bond. This movie has been made and released on the heels of Batman and Star Wars prequels.
- Le Chiffre is a French word which translates into English as "The Cypher" or "The Number".
- In the opening sequence the shape that clubs symbol convolute to is called a Mandelbrot Set in Chaos Theory. It has infinite complexity.
The free running/parkour sequence was inspired by an edited-out roof-top chase sequence from On Her Majesty's Secret Service.
- The number on Dimitrios' key ring was 53. 1953 was the year when Ian Fleming wrote the Casino Royale novel.
- Daniel Craig was Barbara Broccoli's first choice for the role of Bond. She had him in mind after seeing his performance in Layer Cake (2004).
- The movie had the most BAFTA Award nominations ever received by a Bond movie, totaling nine. The previous record had been two each for GoldenEyeand The Spy Who Loved Me. The 9 Nominations were for Best British Film, Adapted Screenplay, Actor in a Leading Role, Film Music, Cinematography, Editing, Production Design, Sound and Special Visual Effects. It only won best sound.
- Vesper Lynd's official job was that of International Liaison Officer for the Financial Action Task Force of HM Treasury. Her work address was 1 Horse Guards Road, London, SW1A 2HQ. Like the use of the real Vauxhall Cross address of Mi6 in the Bond films of the last decade, this is the real address of Her Majesty's Treasury.
- When James Bond sends resignation letter there are several e-mail addresses are seen in his e-mail client. These addresses contain names of computers related personnel involved in making of the film (Anne Bennett, Chris McBride, Robert Wright, etc.).
- Although the password that James Bond enters on the alpha-numeric keypad at the Casino Royale was supposed to be VESPER (837737), the password Bond enters is 836547.
- In one afternoon's shooting, three Aston Martin DBS cars valued at $300,000 each were destroyed for the car roll sequence.
- In the shower scene, Vesper was originally scripted to be wearing nothing but her underwear. Daniel Craig argued that Vesper would not have stopped to take her clothes off, and the scene was changed.
- The car barrel-roll stunt by the Aston Martin DBS broke the world record for the most barrel rolls assisted by a cannon. Originally, the racing specifications of the DBS meant that a standard ramp would not be sufficient to get the car to roll, so the special effects team were called in to install a air-powered cannon behind the drivers seat. This allowed the car to complete seven full rolls. The stunt was officially entered into the Guinness Book of World Records on 5th November 2006.
- Authorities in Venice granted producers permission to sail James Bond's yacht, called Spirit 54, along the Grand Canal between the Accademia and Rialto bridges. No-one can remember exactly the last time a pleasure yacht sailed in the Grand Canal, but it's believed to have been several centuries ago.
- As of its release, Casino Royale had the the longest running time of any Bond movie at 144 minutes.